Cloned (Comment) | Organism |
---|---|
gene FgSAT or FGSG_00186, DNA and amino acid sequence determination and analysisphylogenetic analysis and tree, real-time PCR expression analysis, genetic complementation of FgSAT deletion mutant | Fusarium graminearum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of three deletion mutants of FgSAT, DELTAFgSAT-1, -2 and -18, using a gene replacement strategy. The three mutants show no recognizable phenotypic changes on potato dextrose agar medium, but exhibit a very weak growth on fructose gelatin agar medium containing sulfate as sole sulfur source. Supplementation of O-acetylserine, cysteine, or methionine, but not serine, rescues the defect of mycelial growth in FgSAT deletion mutants. The three mutants have a decrease in conidiation in mung bean liquid, but not in carboxymethyl cellulose. The mutant virulence, deoxynivalenol production and fungicide sensitivity is not altered compared to the wild-type. Real-time PCR assays detect an increase in expression levels of cysteine synthase (FgOAS), cystathionine gamma-synthase (FgCGS), cystathionine beta-lyase (FgCBL) genes involved in the alternative pathway in FgSAT deletion mutants, suggesting that the alternative pathway in Fusarium graminearum is present and can operate. Phenotype, overview | Fusarium graminearum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Fusarium graminearum | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Fusarium graminearum PH-1 | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium graminearum | I1R9N5 | gene FGSG_00186 | - |
Fusarium graminearum PH-1 | I1R9N5 | gene FGSG_00186 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Fusarium graminearum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Fusarium graminearum | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Fusarium graminearum PH-1 | CoA + O-acetyl-L-serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAT | - |
Fusarium graminearum |
General Information | Comment | Organism |
---|---|---|
metabolism | serine O-acetyltransferase (SAT) is a key enzyme converting serine into O-acetylserine in the synthesis of sulfur-containing amino acids | Fusarium graminearum |